GC-MS/MS Analyses of Biological Samples in Support of Developmental Toxic Effects on Percutaneous Exposure of Rats to VX

Abstract

This report documents the results of gas chromatography-tandem mass spectrometry (GC-MS/MS) analyses of blood, tissues, and organs (heart, lung, liver, kidney, brain, eye, diaphragm, and skin) that were obtained from rats (postnatal days 42 and 70) percutaneously exposed to various concentrations of O-ethyl S-(2-diisopropylaminoethyl) methylphosphonothioate (VX). The amount of O-ethyl methylphosphonofluoridate (VX-G) present in these biological samples was quantified using chemical ionization mass spectrometry with isotope dilution. In addition, free VX present on the skin surface was quantified from cellophane tape strip sampling using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2016
Accession Number
AD1012853

Entities

People

  • Christopher E. Byers
  • Jeffrey M. Mcguire
  • Linnzi K. Wright
  • Lucille A. Lumley
  • Michael W. Busch
  • Paul S. Demond

Organizations

  • Edgewood Chemical Biological Center

Tags

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Biomedical Research
  • Cellophane
  • Chemical Warfare
  • Chemical Warfare Agents
  • Chromatography
  • Electrospray Ionization
  • Gas Chromatography
  • Health Services
  • Ionization
  • Liquid Chromatography
  • Mass Spectra
  • Mass Spectrometry
  • Materials
  • Medical Personnel
  • Nerve Agents
  • Spectrometry

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Toxicology/Environmental Toxicology