Defining Key Entry Events for Crimean-Congo Hemorrhagic Fever Virus in Mammalian Cells

Abstract

Crimean Congo hemorrhagic fever virus (CCHFV) is an arthropod-borne virus in the genus Nairovirus within the family Bunyaviridae that causes significant morbidity and mortality in humans. Little is known about CCHFV-host cell interactions, in part due to the requirement for Biological Safety Level 4 containment for studies using infectious virus. Entry studies on other members of the family Bunyaviridae indicate that most of the viruses enter by clathrin-mediated endocytosis (CME). However, a clathrin-independent mechanism was described for one virus within the family, which was a cell-dependent phenomenon. All bunyaviruses that have been studied also require endosomal acidification for entry, with some entering through early endosomes (EE) and others through late endosomes (LE).The goal of this study was to define key cellular entry events for CCHFV.

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Document Details

Document Type
Technical Report
Publication Date
Aug 10, 2012
Accession Number
AD1013095

Entities

People

  • Aura R. Garrison

Organizations

  • Uniformed Services University of the Health Sciences

Tags

DTIC Thesaurus Topics

  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Health Services
  • Lymphocytes
  • Proteins
  • Viruses

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Virology (or Medical Virology).