Generation and Characterization of a Double Recombinant Monkeypox Virus for use in Animal Model Development and Therapeutic Evaluation
Abstract
Monkeypox virus (MPXV) is an emerging/re-emerging zoonotic infection endemic to Central Africa that produces a human disease similar to smallpox. Since the cessation of active vaccination and the global eradication of smallpox, monkeypox has emerged as a potential biological threat due to the vast number of unvaccinated individuals worldwide. Given the adverse events associated with current vaccination strategies, and a lack of fully licensed therapeutic options, there has been sustained interest in countermeasure development, testing, evaluation, and implementation. The continued development of animal models to replicate human disease has been crucial for therapeutic evaluation, and while no current model recapitulates all the features of human disease, nonhuman primate models have come close. Here, we describe the construction of a new double recombinant fluorescent monkeypox virus (MPXV-GFP-tdTR), validate its use for therapeutic evaluation in vitro, and demonstrate its pathogenicity in nonhuman primates. The data support the prospective use of this virus for future animal model development and therapeutic evaluation studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 27, 2012
- Accession Number
- AD1013119
Entities
People
- Kenny L. Lin
Organizations
- Uniformed Services University of the Health Sciences