Protective Effects of Fluoride and Amorphous Calcium Phosphate Against Acid Erosion
Abstract
Background: Erosion is an increasing dental health challenge due to the irreversible effects of enamel loss. Fluoride (F) is a proven therapy for its remineralization effects. Recent studies have suggested that CPP-ACP (casein phosphopeptide-amorphous calcium phosphate) may provide similar protection against acid erosion. Objective: The aim of this pilot study was to compare the enamel-protective effects of fluoride and CPP-ACP against erosive challenges that simulate prolonged exposure to acidic conditions. Methods: Ten de-identified extracted human teeth were sectioned and sanded into 100-micron sections, then embedded along with TEM grids as geometric makers in epoxy to expose a flat enamel edge. A total of 75 specimens were subjected to a daily pH-cycling challenge, alternating between artificial saliva (pH 7.0; 23.5 hours) and citric acid (pH 3.9; 30 minutes). Four concentrations of protective agents (900 ppm F, 5000 ppm F, CPP-ACP, and CPP-ACP + 900 ppm F; control = no treatment) were applied immediately following the acidic challenge at three time intervals (weekly, 3 times per week, and daily applications; n=5 specimens per treatment group/frequency). Microradiographs were taken before experimentation (baseline) and following 1 week and 2 weeks of acid exposure/treatment. Radiographs were digitized and viewed under a stereomicroscope to quantify enamel surface erosion. Results: The results demonstrated that daily applications of various F and ACP therapies were more protective than 3 times per week and weekly applications (two-way ANOVA; P<0.05). Fluoride 5000 ppm applied daily was most effective against acid erosion. The combination of fluoride and CPP-ACP did not appear to improve the protective effects of either agent alone. Conclusions: Based on the results of this pilot study, it may be beneficial to prescribe fluoride 5000 ppm for daily application to reduce the risk of dental erosion.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2012
- Accession Number
- AD1013136
Entities
People
- Thu N. Luu
Organizations
- Uniformed Services University of the Health Sciences