eDNA Barcoding: Using Next-Generation Sequencing of Environmental DNA for Detection and Identification of Cetacean Species

Abstract

We are developing next-generation sequencing and digital (d)PCR methodology for detection and species identification of cetaceans using environmental (e)DNA collected from seawater. Referred to here as (e)DNA barcoding, this new methodology can be used to [a] detect and identify the presence of (non-vocal) cetaceans in the field and [b] identify the species of unknown cetacean vocalizations to improve the accuracy of ongoing U.S. Navy passive acoustic monitoring efforts.

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Document Details

Document Type
Technical Report
Publication Date
Sep 30, 2015
Accession Number
AD1013945

Entities

People

  • Scott Baker

Tags

DTIC Thesaurus Topics

  • Acoustic Detection
  • Cetaceans
  • Controlled Environment
  • Demographic Cohorts
  • Detection
  • Dna Sequence Analysis
  • Filtration
  • Identification
  • Ocean Environments
  • Oceanography
  • Oceans
  • Odontocetes
  • Puget Sound
  • Quality Control
  • Sampling
  • Surface Waters
  • Water

Fields of Study

  • Environmental science

Readers

  • Marine Mammal Biology
  • Oncology and Biomarker-Based Cancer Detection.