Targeting Tryptophan Catabolism: A Novel Method to Block Triple-Negative Breast Cancer Metastasis

Abstract

Triple negative breast cancer (TNBC) cells strongly upregulate two related pathways, the kynurenine pathway (KP) in forced suspension culture. The KP is the principal route of tryptophan catabolism. Interestingly, the intermediate tryptophan metabolite kynurenine (Kyn) was recently identified as an endogenous ligand for AhR, a transcription factor that was also upregulated in suspension. Kyn activation of AhR promotes motility of glioma cells, and increased AhR expression and activity was found to be a characteristic of human and mouse mammary tumors. AhR is also in many immune cell types and its activation decreases T-cell activity leading to tumor immune escape. Since the rate limiting enzyme TDO2 increases in TNBC, the goal of our proposal is to determine if we can target this pathway that helps TNBC metastasize. Our hypothesis is that upregulation of kynurenine by TNBC facilitates survival in transit to metastatic sites and immune suppression and thereby mediates the highly metastatic nature of this subtype. We now have one published work and work in progress suggests that this hypothesis holds true.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2016
Accession Number
AD1014833

Entities

People

  • Jennifer K Richer

Organizations

  • University of Colorado Boulder

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Blood
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Media
  • Culture Techniques
  • Gene Expression
  • Liquid Chromatography
  • Lymphocytes
  • Medical Personnel
  • Metabolism
  • Neoplasms
  • Proteins
  • Transcription Factors

Fields of Study

  • Biology

Readers

  • Battery Technology and Engineering
  • Molecular Biology and Genetics
  • Oncology