Building a Better Mousetrap 1: Using Design of Experiments with Unconfounded Ions to Discover Superior Media for Growth and Lipid Production by Chlorella sp. EN1234

Abstract

An unconfounded Scheffe Mix approach was used to probe important ions and their interactions in supporting biomass and lipid production by Chlorella sp. EN1234. Six major cations and anions; NH4+, NO3-, Na+, K+, PO4- and Cl-; were investigated. Piepel plots and RSM analysis showed that in a number of cases, the major media anions PO4- and Cl- negatively influence final cell densities, and that maximal cell density is obtained with nitrate over ammonium, with an optimal effect when mixed with equal molar potassium. Although it is commonly assumed that lipid content increases in nitrogen-deficient media, here little correlation was found between nitrogen content and total lipid content with mixtures that supported high lipid productivity. Thus these mixtures define the composition space within which further R and D might produce that best trade-off between total biomass production and high cellular lipid content.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2014
Accession Number
AD1015539

Entities

People

  • Donald Veverka
  • Megan Mraz
  • Melanie Grogger
  • Patrick C Hallenbeck

Organizations

  • United States Air Force Academy

Tags

DTIC Thesaurus Topics

  • Algae
  • Cells
  • Data Science
  • Elements
  • Experimental Design
  • Information Science
  • Nitrogen
  • Potassium
  • Production
  • Productivity

Readers

  • Analytical Chemistry
  • Marine Ecotoxicology
  • Regression Analysis.

Technology Areas

  • Space