Factors Essential for Prostate Cancer Metastasis Revealed Through a Novel 3D Microtissue Assay

Abstract

Growing evidence suggest that the micro-dissemination of bone metastatic prostate cancer (BMPCa) to bone marrow (BM) may be facilitated in early-stage of cancer development and could be the origin of future outgrowth and metastasis. Importantly, the osteoblastic niche is known to constitute the sanctuary for bone-homing cancer cells where it confers BMPCa dormancy and therapy-resistance. Under the support of current proposal, we have carefully validated a novel 3D osteoblastic niche (3D-ObN) assay system which recapitulate BMPCa and osteoblastic niche interactions. Throughout the research year we have made following progressions.1) We verified that the 3D-ObN can recruit BMPCa as well as other metastatic cancers.2) We conducted a novel application of Hypoxia-sensitive reporters to measure BMPCa invasion ability and to apply for HTS.3) 3D-ObN recruits cancer cells depending on high metastatic ability, cancer stem-like subsets, and CXCR4-CXCL12 interaction.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2016
Accession Number
AD1016881

Entities

People

  • Seunghwan Lim

Organizations

  • Case Western Reserve University

Tags

DTIC Thesaurus Topics

  • Acquisition
  • Biomedical Research
  • Bone Marrow
  • Breast Cancer
  • Cell Line
  • Cells
  • Confocal Microscopy
  • Culture Techniques
  • Department Of Defense
  • Medical Personnel
  • Microscopy
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Stem Cells
  • Therapy
  • Three Dimensional

Readers

  • Oncology (Cancer Research).