Comparison of Topical Hemostatic Agents in a Swine Model of Extremity Arterial Hemorrhage: BloodSTOP iX Battle Matrix vs. QuikClot Combat Gauze

Abstract

BloodSTOP iX Battle Matrix (BM) and QuikClot Combat Gauze (CG) have both been used to treat traumatic bleeding. The purpose of this study was to examine the efficacy and initial safety of both products in a swine extremity arterial hemorrhage model, which mimics combat injury. Swine (37.13 0.56 kg, NBM = 11, NCG = 9) were anesthetized and splenectomized. We then isolated the femoral arteries and performed a 6-mm arteriotomy. After 45 s of free bleeding, either BM or CG was applied. Fluid resuscitation was provided to maintain a mean arterial pressure of 65 mmHg. Animals were observed for 3 h or until death. Fluoroscopic angiography and wound stability challenge tests were performed on survivors. Tissue samples were collected for histologic examination. Stable hemostasis was achieved in 11/11 BM and 5/9 CG subjects, with recovery of mean arterial pressure and animal survival for 3 h [p <0.05, odds ratio (OR) = 18.82 (0.85415.3)]. Time to stable hemostasis was shorter for the BM-treated group (4.8 2.5 min vs. 58 20.1 min; median = 2, interquartile range (IQR) = 0 min vs. median = 60, IQR = 120 min; p < 0.05) and experienced longer total stable hemostasis (175.2 2.5 min vs. 92.4 29.9 min; median = 178, IQR = 0 min vs. median = 120, IQR = 178 min; p < 0.05). Post treatment blood loss was lower with BM (9.5 2.4 mL/kg, median = 10.52, IQR = 13.63 mL/kg) compared to CG (29.9 9.9 mL/kg, median = 29.38, IQR = 62.44 mL/kg) (p = 0.2875). Standard BM products weighed less compared to CG (6.9 0.03 g vs. 20.2 0.4g) (p < 0.05) and absorbed less blood (3.4 0.8 g vs. 41.9 12.3 g) (p < 0.05). Fluoroscopic angiography showed recanalization in5/11 (BM) and 0/5 (CG) surviving animals [p = 0.07, OR = 9.3 (0.41208.8)].

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Document Details

Document Type
Technical Report
Publication Date
Apr 12, 2016
Accession Number
AD1017181

Entities

People

  • Amanda Reed-maldonado
  • Amjad Alwaal
  • Guiting Lin
  • Huixi Li
  • Lia Banie
  • Lin Wang
  • Reginald B. O'hara
  • Taylor A. Spangler
  • Yung-chin Lee

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Arteries
  • Blood
  • Blood Coagulation
  • Cardiovascular Physiological Phenomena
  • Cardiovascular System
  • Combat Injuries
  • Health Services
  • Medical Personnel
  • Surgery
  • Veins

Fields of Study

  • Medicine

Readers

  • Mathematics or Statistics
  • Toxicology/Environmental Toxicology
  • Trauma or Military Medicine