Enhancing the Breadth and Efficacy of Therapeutic Vaccines for Breast Cancer

Abstract

The focus of the Spellman/Gray work group during the research period has been upon the generation of materials, tools, and data for the purpose of aiding and supporting the research and findings of the entire multi-team collaboration endeavoring to identify antigenic targets for breast cancer-infiltrating T cells. Our team has achieved a number of accomplishments. We have determined the likely specificity of immunogenic peptides for MHC alleles from a collection of MHC-I-bound epitopes eluted from the cell surface of twenty breast cancer cell lines. We developed computational pipelines to identify the sequence of the complete TCR heterodimer was applied to new samples sequenced by our colleagues in Denver. We also developed an RNAseq pipeline to identify likely neoantigens in breast cancer using public data.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2016
Accession Number
AD1018944

Entities

People

  • Dmitri Rosanov
  • Kami Chiotti
  • Paul Spellman

Organizations

  • Oregon Health & Science University

Tags

DTIC Thesaurus Topics

  • Antigens
  • Blood
  • Breast Cancer
  • Carrier Proteins
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Computational Biology
  • Data Sets
  • Gene Expression
  • Genetics
  • Lymphocytes
  • Neoplasms
  • Proteins
  • Proteomics

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech