Single-Cell RNA Sequencing of the Bronchial Epithelium in Smokers With Lung Cancer

Abstract

Over the next year we plan to sequence between 100 and 200 cell per donor from 12 former smokers and 24 current and former smokers undergoing bronchoscopy for the suspicion of lung cancer. These samples will allow us to characterize the cell populations upon smoking cessation, in high-risk smokers without lung cancer, and in smokers with lung cancer. These data will provide a comprehensive single cell transcriptional profile of changes that occur in the bronchial epithelium in response to smoking, smoking cessation, and lung cancer. These discoveries may enhance current lung cancer diagnostics as well as suggest potential new therapeutics for lung cancer.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2016
Accession Number
AD1020692

Entities

People

  • Jennifer Beane-ebel

Organizations

  • Boston University School of Medicine

Tags

DTIC Thesaurus Topics

  • Biological Markers
  • Blood Cells
  • Bronchoscopy
  • Cells
  • Data Analysis
  • Department Of Defense
  • Epithelial Cells
  • Epithelium
  • Gene Expression
  • Health Services
  • Lung Cancer
  • Medical Personnel
  • Neoplasms
  • Nucleic Acids
  • Pipelines
  • Rna Sequence Analysis
  • Tissues

Fields of Study

  • Biology

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