Development of a Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS) Method for the Quantitation of Viral Envelope Glycoprotein in Ebola Virus-Like Particle Vaccine Preparations

Abstract

Ebola virus like particles; (EBOV VLPs, eVLPs), are produced by expressing the viral transmembrane glycoprotein (GP) and the structural matrix protein VP40 in mammalian cells. When expressed, these proteins self-assemble and bud from host cells displaying morphology similar to infectious virions. Several studies have shown that rodents and non-human primates vaccinated with eVLPs are protected from lethal EBOV challenge. The mucin-like domain of envelope glycoprotein (GP1) serves as the major target for a productive humoral immune response. Therefore GP1 concentration is a critical quality attribute of EBOV vaccines and accurate measurement of the amount of GP1 present in eVLP lots is crucial to understanding variability in vaccine efficacy.

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Document Details

Document Type
Technical Report
Publication Date
Sep 05, 2016
Accession Number
AD1021613

Entities

People

  • Chris Mahone
  • Ernie Brueggemann
  • Jonathan E. Nuss
  • Karen A Martins
  • Lisa H Cazares
  • Michael D. Ward
  • Paul Demond
  • Sina Bavari
  • Tara Kenny
  • Trevor Glaros

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Accuracy
  • Amino Acids
  • Chemistry
  • Detection
  • Ebola Virus
  • High Resolution
  • Liquid Chromatography
  • Mass Spectrometry
  • Materials
  • Measurement
  • Papillomavirus Infections
  • Rodents
  • Spectrometry
  • Vaccines
  • Viral Structures
  • Virion
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Infectious Disease/Epidemiology

Technology Areas

  • Biotechnology
  • Space