In Situ Bioremediation of 1,4-Dioxane by Methane Oxidizing Bacteria in Coupled Anaerobic-Aerobic Zones
Abstract
Microcosm tests, microbial enrichments, pure culture studies, and column experiments were performed to evaluate the co-metabolic biodegradation of 1,4-dioxane ( 1,4-D) by methane-, ethane- and propane-oxidizing bacteria. Results obtained during this limited scope study showed that methane-oxidizing bacteria were not effective for promoting the biodegradation of 1,4-D. For all conditions examined, including use of copper chelators, various nutrients, pure and enrichment cultures, and soil collected from 3 different sites, 1,4-D biodegradation was not observed with methane as a cosubstrate. However, ethane- and propane-oxidizing bacteria were shown to biodegrade 1,4-D. Michaelis-Menten kinetic parameters were determined for both 1,4-D and ethane during degradation by an enrichment culture. Regression of the model parameters Vmax and Km for 1,4-dioxane were 4.6 +/- 1.7 x 10(-5) mg/mg/cell/hand 0.23 +/- 0.07 mg/L, respectively; Vmax and Km for ethane were 1.8 +/- 0.6 x 10(-3) mg/mg/cell/hand 0.064 +/- 0.02 mg/L, respectively. Overall, the data suggest that ethane and propane are more effective cosubstrates than methane for stimulating 1,4-D biodegradation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 11, 2016
- Accession Number
- AD1022557
Entities
People
- Charles E Schaefer
- Paul B. Hatzinger
- Paul K. Van Groos