Early Identification of Molecular Predictors of Heterotopic Ossification Following Extremity Blast Injury with a Biomarker Assay

Abstract

The purpose of this project is to identify predictive markers of heterotopic ossification in an established animal model that would forecast development of heterotopic ossification(HO) in humans soon after injury. Blast procedures have been completed on all 30 animals(Groups I and II) in the year 1 SOW and 45 animals (Groups III V) in the year 2 SOW. All animals were biopsied and have been sacrificed according to protocol schedule. Groups I and II animals were also followed with scheduled routine radiographs to monitor progression of HO. Specimen samples are under analysis for gene and protein level expression with the Nesti partnering molecular biology lab. In the upcoming year, early-appearing gene and protein biomarkers will be identified by analyzing correlation in animals exhibiting radiographic evidence of HO and will be compared to gene expression signatures in existing human tissue samples known to have gone on to develop HO by the partnering lab.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2016
Accession Number
AD1024132

Entities

People

  • Alexander M. Chiaramonti
  • Vincent Jr D. Pellegrini

Organizations

  • Medical University of South Carolina

Tags

DTIC Thesaurus Topics

  • Amputation
  • Biological Markers
  • Blast Injuries
  • Data Analysis
  • Diseases And Disorders
  • Gene Expression
  • Heterotopic Ossification
  • Identification
  • Medical Personnel
  • Military Medicine
  • Osteogenesis
  • Prosthetics
  • Residual Limbs
  • South Carolina
  • Surgical Amputations
  • Tissues
  • United States

Fields of Study

  • Biology

Readers

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  • Trauma Surgery or Emergency Medicine.