Monitoring of Viral Induced Cell Death Using Real Time Cell Analysis

Abstract

The TCID50 is a measure of infectious viral titre in a sample. Traditionally this assay has been carried out using a microscopy based assay. This assay is highly subjective and relies on the correct identification of cytopathic effect (CPE) by eye. In order to ascertain whether the xCELLigence real-time cell analysis (RTCA) platform could be used to deliver a more objective and robust measure of TCID50, a comparative study of the two methods was carried out. Two viruses were analysed, Gan Gan and Holmes Jungle viruses, members of family Bunyaviridae and Rhabdoviridae respectively. The RTCA method gave results very close to the traditional method and was significantly quicker and easier to analyse. The RTCA method also has the potential to provide other information about the viral infection of cells which if explored further could be useful.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2016
Accession Number
AD1024194

Entities

People

  • David Thirkettle-watts
  • Penny Gauci

Organizations

  • Defence Science and Technology Group

Tags

DTIC Thesaurus Topics

  • Arbovirus Infections
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Cultured Cells
  • Deoxyribonucleic Acids
  • Detection
  • Diseases And Disorders
  • Equine Encephalitis
  • Impedance
  • Infection
  • Infectious Diseases
  • Medical Personnel
  • Rna Viruses
  • Virology
  • Viruses

Fields of Study

  • Biology

Readers

  • Naval Mine Countermeasure Systems Development.
  • Systems Analysis and Design
  • Virology (or Medical Virology).