Studies of the Effects of Perfluorocarbon Emulsions on Platelet Number and Function in Models of Critical Battlefield Injury
Abstract
Perfluorocarbon emulsions (PFCs) can treat traumatic injuries (traumatic brain injury (TBI), hemorrhagic shock and burns by enhanced delivery of oxygen. A class-based side effect of PFC (day 2-5 after infusion in 30-50%) may be thrombocytopenia (TCYP). The mechanism is inadequately investigated. The US Food and Drug Administration (FDA)requests investigation of the phenomenon to exclude platelet inflammatory/embolic safety risks. The initial results (phase I) showed that PFC infusion in the normal sheep did not significantly change the platelet number and activation among the experimental and control groups. In 2015 (Phase II and III), PFC infusion as a part of resuscitation fluid was used in sheep with hemorrhage (n=39) and polytrauma (blast traumatic injury and hemorrhage, n=8). The results showed that the sheep's platelet count and fibrinogen level were reduced immediately after resuscitation. However, there was no significant change of platelet number and activation after PFC infusion compared with non-PFC controls over the 7 survival days. Platelet contractile force (PCF, Platelet activator) also showed no significant change compared with control groups (saline and surgical control). Platelet morphological observation corresponded well with function assays. There were no significant percentage changes in neutrophils and monocytes after PFC infusion in injured sheep.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2016
- Accession Number
- AD1024714
Entities
People
- Bruce D Spiess
Organizations
- Virginia Commonwealth University