Targeting Histone Abnormality in Triple Negative Breast Cancer

Abstract

In this funding period, we continued to test the hypothesis that silencing of key tumor suppressive genes by enhanced crosstalk between LSD1 and HDAC5 is a unique epigenetic mechanism promoting TNBC growth, and blockade of the HDAC5-LSD1 axis results in profound inhibition of TNBC growth and metastasis. By using In vitro pull-down assays with His-tag recombinant LSD1 protein incubating withHDAC5 full-length or deletion mutants and immunofluorescence assays, we identified that HDAC5 domain containing nuclear localization element is essential for interaction with LSD1.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2016
Accession Number
AD1024764

Entities

People

  • Yi Huang

Organizations

  • University of Pittsburgh

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Carcinoma
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Enzyme Inhibitors
  • Health Services
  • Medical Personnel
  • Neoplasms
  • Oncology
  • Proteins
  • Students
  • Therapy

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Neuroscience
  • Oncology (Cancer Research).