Acquisition of a Circular Dichroism Spectrometer to Study Biological Molecules at Interfaces
Abstract
We have made substantial progress in the design, implementation, and use of our purchased Circular Dichroism (CD) system and the humidity control device. Currently this CD spectrometer can be used to study peptide/proteins in solution, on surface, and at different humidity levels. We successfully set up a humidity control system which can control the humidity of the CD sample compartment. Humid air is harmful to the optical components of the CD spectrometer. Therefore instead of building a humidity control system inside the CD spectrometer, we built an air tight chamber in the sample compartment, a humid air balance box with humidity control device outside of the CD spectrometer. We connected these two parts using light resistant tubes. We successfully applied this newly installed CD system to study various surface immobilized peptides and proteins. We successfully studied secondary structures of surface immobilized peptides/proteins with different surface immobilization methods, on different immobilization substrates, at different environmental conditions, and with or without co-immobilized hydromimetic functionalities.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 10, 2016
- Accession Number
- AD1024842
Entities
People
- Zhan Chen
Organizations
- University of Michigan