Amplifying Riboswitch Signal Output using Cellular Wiring
Abstract
If fieldable riboswitch-based biological sensors are to fulfill their potential, it is necessary to increase their signal output. Here we report a novel modular amplification system using a riboswitch to initiate signaling between a sensing strain and a reporter strain of E. coli. A quorum sensing signaling molecule biologically wires the sensing and reporter strains together. The amplification circuit increased the amount of fluorescence generated on ligand binding compared to when the riboswitch controlled fluorescence expression directly.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 30, 2017
- Accession Number
- AD1030123
Entities
People
- Annastacia C. Bennett
- Brenton R. Jennewine
- Emily Briskin
- Michael S Goodson
- Nancy Kelley-Loughnane
- Svetlana V Harbaugh
Organizations
- Universal Energy Systems