Entrapment of Bacteriocin 105B onto Fabric with Titania
Abstract
Current methods to fabricate multifunctional textiles to provide multiple facets of protection to the Warfighter are restrictive and not efficient. To address this challenge, previous work by the FORCE ProTex effort encapsulated a narrow-spectrum antimicrobial, the bacteriocin nisin, in a photocatalytic matrix, titanium dioxide (titania), on fabric to evaluate the creation of a multifunctional textile in unison. This report summarizes preliminary investigations to leverage this work by employing a different bacteriocin, an isolate from Bacillus anthracis termed 105b, to fabricate a multifunctional textile exhibiting an alternative range of antimicrobial activity from that of nisin, by titania encapsulation of 105b onto fabric. The results of these initial studies suggest that both pure preparations and semi-pure preparations of 105b are active when encapsulated in titania in solution. However, when the pure preparation of 105b is titania encapsulated on fabric, antimicrobial activity is not observed. It is hypothesized that the lack of activity of encapsulated pure 105b may be due to the removal of molecular stabilizers during the purification process that are responsible for maintaining the stability of the peptide, rendering it inactive. Further studies are necessary to confirm this mechanism. Additional work is also required to retain the activity of encapsulated pure 105b, ascertain the photocatalytic activity of the titania matrix, as well as elucidate the conditions for gradual release of the bacteriocin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 09, 2017
- Accession Number
- AD1030304
Entities
People
- Jennifer M. Rego
- Robert Stote
Organizations
- United States Army Soldier Systems Center