Msi2 Regulates the Aggressiveness of Non-Small Cell Lung Cancer (NSCLC)
Abstract
Purpose, scope: The objective of this project is to expand our mechanistic data to characterize the functional roles of MSI2 in human NSCLC cells and test whether MSI2-overexpressing cells are more sensitive to gamma-secretase and TGF-beta receptor Type I kinase (TGF-beta RI) inhibitors (Aim 1). I also aim to investigate if MSI2 expression is clinically predictive in tumor specimens from lung cancer patients (Aim 2). Major findings and progress: Depletion of MSI2 in multiple independent metastatic murine and human NSCLC cell lines reduced invasion and metastatic potential, independent proliferation effects. MSI2 depletion significantly induced expression of proteins associated with epithelial identity, including tight junction claudin proteins and down-regulated direct translational targets associated with EMT, and unexpectedly upregulated NOTCH pathways. Depletion of TGF-beta RI or SMAD3 resulted in reduced invasion, while overexpression of TGF-beta RI reversed the loss of invasion associated with MSI2 depletion. Interestingly, MSI2 depletion reduced E-cadherin expression, while increasing fibronectin (FN1), reflecting a mixed epithelial-mesenchymal phenotype. MSI2 provides essential support for TGF-beta RI/SMAD3 signaling, contributes to NSCLC progression and may be a predictive biomarker of NSCLC aggressiveness. TGF-beta RI and gamma-secretase drug studies in vitro and in vivo are ongoing, while immunohistochemistry studies are starting Fall 2016.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2016
- Accession Number
- AD1030544
Entities
People
- Yanis Boumber
Organizations
- University of New Mexico