Generation and Characterization of Protective Antibodies to Marburg Virus

Abstract

Marburg virus (MARV) and Ebola virus (EBOV) have been a source of epidemics and outbreaks for several decades. We present here the generation and characterization of the first protective antibodies specific for wild-type MARV. Non-human primates (NHP), cynomolgus macaques, were immunized with viral-replicon particles expressing the glycoproteins (GP) of MARV (Ci67 isolate). An antibody fragment (single chain variable fragment, scFv) phage display library was built after four immunogen injections, and screened against the GP1-649 of MARV. Sequencing of 192 selected clones identified 18 clones with distinct VH and VL sequences.

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Document Details

Document Type
Technical Report
Publication Date
Apr 03, 2017
Accession Number
AD1031198

Entities

People

  • A. Z. Wec
  • J. M. Brannan
  • J. M. Dye
  • Jeffrey Ii W. Froude
  • K. Chandran
  • M. Hust
  • P. Thullier
  • R. R. Bakken
  • S. E. Zak
  • S. Miethe
  • T. Pelat

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Blood
  • Cells
  • Ebola Virus
  • Encephalitis
  • Equine Encephalitis
  • Genetic Structures
  • Health Services
  • Infectious Diseases
  • Microbiology
  • Proteins
  • Rna Virus Infections
  • Rna Viruses
  • Surface Plasmon Resonance
  • Therapy
  • Viral Hemorrhagic Fevers
  • Virus Diseases
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Infectious Disease/Epidemiology
  • Molecular Genetics

Technology Areas

  • Space