Synergic Investigation Of The Self-Assembly Structure And Mechanism Of Retroviral Capsid Proteins By Solid State NMR, Transmission Electron Microscopy And Multiscale simulation

Abstract

The Rous Sarcoma Virus (RSV) is the ideal platform to study retrovirus. The RSV capsid enclosing the viral genome materials is assembled from ~ 1500 copies of the 237-residue RSV capsid protein (CA). In vitro, tubular assembly can be obtained with the CA with similar underlying structural properties as the authentic RSV capsid. Due to strong polymorphism, RSV CA assemblies are challenging for structural characterization by techniques such as X-ray diffraction or cryo Electron Microscopy (cryo-EM).

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Document Details

Document Type
Technical Report
Publication Date
Mar 29, 2017
Accession Number
AD1031640

Entities

People

  • Bo Chen

Organizations

  • University of Central Florida

Tags

Communities of Interest

  • Air Platforms
  • Energy and Power Technologies
  • Human Systems

DTIC Thesaurus Topics

  • Air Force Research Laboratories
  • Amino Acids
  • Chemical Shifts
  • Crystal Structure
  • Electron Microscopy
  • Electrons
  • Frequency
  • Magnetic Resonance
  • Microscopy
  • Molecular Dynamics
  • Multiscale Simulations
  • Resonance
  • Self Assembly
  • Simulations
  • Transmission Electron Microscopy
  • X Rays
  • X-Ray Diffraction

Fields of Study

  • Chemistry

Readers

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  • Molecular and Cellular Biochemistry
  • Molecular and genetic basis of cancer.

Technology Areas

  • Microelectronics