Intra-Articular Lubricin Gene Therapy for Post-Traumatic Arthritis
Abstract
Twenty-six rabbits underwent sham or ACLT surgery and were euthanized at 4 or 8 weeks post-op, completing live animal work for Major Task 1. Histologic processing was completed and safranin-O-stained sections were scanned. All rabbits that underwent surgery survived to the scheduled endpoints without apparent complications. However, the 10 rabbits euthanized at 4 weeks (5 sham and 5 ACLT) all had joint effusions, indicating an inflammatory reaction to AAV. Effusions were not evident in our initial one-week pilot study designed to detect such responses. A review of AAV titers indicated that the preparation used in the 4-week study was ~ 2-fold more concentrated than that used in the earlier study, suggesting that the effusions were provoked by viral overload. Given these events it would appear prudent to better define the maximum tolerated dose of AAV before beginning Major Task 2, which calls for treatment after OA progression is already underway.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2016
- Accession Number
- AD1032858
Entities
People
- James A. Martin
Organizations
- University of Iowa