Homeodomain Interacting Protein Kinases Modulate Hypoxic Adaptation and Chemoresistance
Abstract
The goal of this project was to dissect the roles of Home domain Interacting Protein Kinases(HIPK) isoforms 2 and 3 in the development of castration resistant prostate cancer (PCa) following hypoxic reprogramming using established cell lines. Our objectives were to (1)ectopically express or silence HIPK2 and HIPK3 in PCa cell lines (LnCAP and LnCAP-abl) , (2)define the impact of HIPK manipulation on PCa cell biology (proliferation, apoptosis, metabolism, etc.), (3) evaluate the interplay of HIPK2 and HIPK3 with the Hippo signaling pathway, and (4) delineate the roles of HIPK2 and HIPK3 in PCa chemo-resistance. Our studies were able to demonstrate that HIPK2 was androgen responsive and under direct control of the androgen receptor. We were also able to clone HIPK2 and HIPK3 into expressing vectors and obtain small RNAs for silencing. Technical difficulties prevented our group from evaluating the impact on HIPKs in PCa as ectopic expression produced a protein of incorrect size and silencing proved ineffective. We will resolve these issue and continue to examine HIPKs inPCa in future studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2015
- Accession Number
- AD1032888
Entities
People
- Jeremy W. Chambers
- Myles Hodgson
Organizations
- Florida International University