Integration of Peptides into Organic Thin Film Transistor (OTFT)-based Printable Sensors
Abstract
Biosensors based on organic thin film transistor technology offer the exciting prospect of detecting and identifying peptides; biomarkers found in bodily fluids. This development would assist in the monitoring or identification of medical diseases and facilitate rapid, point-of-care testing. In particular, neuropeptide Y is a highly abundant neurotransmitter with links to many important physiological functions, including stress response. Its rapid detection provides an opportunity to identify anxiety and stress related disorders such as post-traumatic stress disorder. Moreover, inkjet printing of organic electronic inks may provide a pathway for reliable, large scale production of low cost sensors. In this project, multiple detection mechanisms for two different peptides, L-reduced glutathione and neuropeptide Y, are presented. A pathway for impedance-based peptide detection using Nafion films was also presented. Receptor elements for glutathione detection were incorporated into the sensor device structure and a current response three times larger than that of water was obtained when 2 mM glutathione was placed on the sensor. This response demonstrated an increase in sensitivity of five times over the non-transistor based sensing mechanism. Electrochemical detection of neuropeptide Y was also achieved, with the method proposed, utilising monoclonal anti-neuropeptide Y antibodies, enabling detection of neuropeptide Y concentrations below 95 nM.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 10, 2017
- Accession Number
- AD1033045
Entities
People
- Paul Dastoor
- Warwick J. Belcher
- Xiaojing Zhou
Organizations
- University of Newcastle