Validation and Interrogation of Differentially Expressed and Alternatively Spliced Genes in African-American Prostate Cancer

Abstract

These studies address the urgent need to elucidate the molecular mechanisms underlying the more aggressive prostate cancer biology in African American (AA) men. Specifically, our objectives are to 1) expand our sample cohort and delineate the relationship between genetic/epigenetic/posttranscriptional factors in AA prostate cancer and Gleason grade and 2) manipulate splicing using novel splice-switching oligonucleotides (SSOs) and determine functional outcomes. Toward these objectives, we have opened our Genomics of Cancer Disparities Study to obtain AA and white prostate cancer blood and tissue specimens. For all tissue specimens collected, we have screened for tumor content, determined Gleason grade, isolated DNA and RNA and annotated. In addition, we have designed and synthesized SSOs to manipulate PIK3CD alternative splicing, with SSOs targeting the exon 23junction or a putative enhancer to produce the AA PIK3CD variant and targeting a putative repressor to produce the white PIK3CD variant.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2016
Accession Number
AD1033187

Entities

People

  • Jennifer Freedman
  • Steven R. Patierno

Organizations

  • Duke University

Tags

DTIC Thesaurus Topics

  • African Americans
  • Androgen Receptors
  • Biology
  • Cancer
  • Department Of Defense
  • Ethnic Groups
  • Genetics
  • Health
  • Medical Personnel
  • Minority Groups
  • Neoplasms
  • Oncology
  • Prostate
  • Prostate Cancer
  • Regression Analysis
  • Skin
  • Therapy

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Oncology and Biomarker-Based Cancer Detection.
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology