Systematic Identification of Genes Required for Expression of Androgen Receptor Splice Variants
Abstract
Recently, a new genetic engineering tool has been developed from bacterial Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated System (Cas). It has several advantages over the existing ones and one of them is the ability to make a very large number (tenth of thousands) of gene knockouts so that we can screen genes important to prostate cancer. For example, androgen receptor (AR) is well known for its role in prostate cancer progression and castration resistance, and several AR splicing variants have been identified in castration resistant prostate cancer. With this technology, we can answer questions like how prostate cancer becomes castration resistant after anti-hormone therapy. Furthermore, these genes screened out with this technology may serve biomarkers or therapeutic targets. Thus, the overall of goal of this application is to identify genes involved in regulation of AR splice variant through CRISPR/Cas screening system.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2016
- Accession Number
- AD1033234
Entities
People
- Yin-Yuan Mo
Organizations
- University of Mississippi Medical Center