Global Identification of Disease Associated Genes in Fragile X Cells
Abstract
The aim of the proposed study is to test the hypothesis that the Fragile X mental retardation protein (FMRP) prevents/resolves R loop formation to maintain genome stability. Specifically we propose that stable R loop formation impedes replication fork progression, resulting in DNA double strand breaks (DSBs), and that FMRP functions to prevent such replication-transcription conflict. We have performed three biological replicate experiments to rigorously test if the Fragile X cell line produces more DSBs than the normal control. We also developed a yeast-based recombination assay to directly test the proposed function of FMRP in R loop prevention/resolution. Finally, we performed a ChIP-seq experiment to identify the chromatin binding sites of FMRP. We are working towards obtaining a short list of genes with overlapping DSBs, R loop forming sites and FMRP-binding sites. Potential disease-correlation of these genes will then be assessed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2016
- Accession Number
- AD1033996
Entities
People
- Wenyi Feng