Development of a Prognostic Marker for Lung Cancer Using Analysis of Tumor Evolution

Abstract

The goal of this project is to sequence the exomes of single tumor cells from tumors in order to construct evolutionary trees, the characteristics of which will be used to predict whether a tumor will metastasize or not. We established a procedure for isolating tumor cells from lung tumor tissue and observed high variability in yield. We also encountered loss of tumor cell viability after freezing. Despite these challenges, we will be able to obtain sufficient numbers of cells to proceed. The next step is whole genome amplification of the DNA from single cells as a prerequisite to sequencing. We encountered a low success rate of whole genome amplification from single sorted cells and have spent a great deal of time troubleshooting this process. We anticipate that exome sequencing of single tumor cells should begin shortly in order to reconstruct tumor cell evolution in tumors.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2016
Accession Number
AD1034019

Entities

People

  • Edward F. Patz

Organizations

  • Duke University

Tags

DTIC Thesaurus Topics

  • Amplification
  • Biomedical Research
  • Blood
  • Blood Cells
  • Cancer
  • Cell Physiological Processes
  • Cells
  • Dissociation
  • Flight Training
  • Freezing
  • Gel Electrophoresis
  • Health Services
  • Lung Cancer
  • Neoplasms
  • Personal Information Managers
  • Sequences
  • Viability

Fields of Study

  • Biology

Readers

  • Molecular and genetic basis of cancer.
  • Oncology
  • Theoretical Analysis.