Activation of Central Pattern Generator for Respiration Following Complete High Cervical Spinal Cord Interruption
Abstract
The original hypothesis was that intraspinal inhibitory circuits (GABA- and Glycinergic) play an important role of in the control of a spinal central pattern generator (CPG) for breathing. These inhibitory spinal interneurons were hypothesized to contribute to suppression of the respiratory CPG in both intact and post-injury (high cervical transection) conditions. Adhering to the experiments outlined in our SOW, spinal respiratory neurons (cervical C3-C5 and C1-C2 levels) were characterized by their location, pattern (via extra- and intracellular recordings) and sensitivity to blockers of GABAa and Glycine receptors (GABAzine and strychnine).CPG-specific inspiratory bursts, recorded from phrenic nerves, were observed after application of GABAzine and strychnine over C3-C5 cervical segments in spinally transected and intact animals (Ghali and Marchenko, 2016). These spinal bursts were not phase-locked to the supraspinal (ponto-medullary) respiratory rhythm. We recorded spinal interneurons related to the spinal respiratory CPG, a promising target for activation of breathing in tetraplegic patients. These newest findings are being prepared for publication in peer-review scientific journals and presentation at the upcoming annual meeting for the Society for Neuroscience.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2016
- Accession Number
- AD1034097
Entities
People
- Vitaliy Marchenko
Organizations
- Drexel University