Multifunctional PSCA Antibody Fragments for PET and Optical Prostate Cancer Imaging
Abstract
The goals of this initial year of funding were to develop engineered antibody fragments (cys-diabodies and cys-minibodies) suitable for labeling with positron-emitting radionuclides and fluorescent dyes for multimodal imaging of prostate cancer. A novel cys-minibody recognizing PSCA (prostate stem cell antigen) has been produced. Conjugation conditions have been developed for radiolabeling with either and I-124 and Zr-89 via lysine (random) or cysteine (site-specific) residues of the proteins. Targeting, imaging, and biodistribution studies in mouse models of prostate cancer confirm high activity in PSCA-positive tumors with excellent visualization by PET imaging. Recently, dual-labeling (with radionuclide and fluorescent dye) of the cys-minibody has been developed and evaluated in mouse models.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2016
- Accession Number
- AD1034662
Entities
People
- Anna M. Wu
Organizations
- University of California