Inactivation of West Nile Virus in Serum with Heat, Ionic Detergent, and Reducing Agent for Proteomic Applications
Abstract
Research involving biosafety level 3 pathogens such as West Nile virus (WNV) is often limited by the limited space and technical constraints of these environments. To conduct complex analytical studies outside of high containment, robust and reliable inactivation methods are needed that maintain compatibility with downstream assays. Here we report the inactivation of WNV in spiked serum samples using a commercially available SDS-PAGE sample buffer for proteomic studies. Using this method, we demonstrate its utility by identification proteins differentially expressed in the serum of mice experimentally infected with WNV.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 14, 2017
- Accession Number
- AD1035782
Entities
People
- Alyssa M. Jespersen
- David A. Kulesh
- David E Harbourt
- Ernst E. Brueggemann
- James G. Jaissle
- Jeff Garrison
- Leonard P. Wasieloski
- Lisa H Cazares
- Louis A Altamura
- Michael D. Ward
- Sundus Ahmed
- Susan R. Coyne
- Tara Kenny
- Timothy D Minogue
Organizations
- United States Army Medical Research Institute of Infectious Diseases