A DNA Vaccine for Crimean Congo Hemorrhagic Fever Protects Against Disease and Death in Two Lethal Mouse Models

Abstract

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus capable of causing a severe hemorrhagic fever disease in humans. There are currently no licensed vaccines to prevent CCHFV infection. We developed a DNA vaccine expressing the M-segment glycoprotein genes of CCHFV and assessed its immunogenicity and protective efficacy in two lethal mouse models of disease: type I interferon receptor knockout (IFNAR-/-) mice; and a novel transiently immune suppressed (IS) mouse model. Vaccination of mice by muscle electroporation of the M-segment DNA vaccine elicited strong antigen-specific humoral immune responses with neutralizing titers after three vaccinations in both IFNAR-/- and IS mouse models.

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Document Details

Document Type
Technical Report
Publication Date
Sep 18, 2017
Accession Number
AD1035792

Entities

People

  • Aura R. Garrison
  • Carolyn M. Six
  • Catherine V. Badger
  • Charles J. Shoemaker
  • Collin J. Fitzpatrick
  • Connie S Schmaljohn
  • Drew Hannaman
  • Eric Bergeron
  • Jacqueline D. Martin
  • Jeffrey W. Koehler
  • John J Suschak
  • Joseph W. Golden
  • Marko Zivcec
  • Michelle J. Richards

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Animals
  • Biological Factors
  • Blood
  • Cells
  • Chemistry
  • Culture Techniques
  • Encephalitis
  • Equine Encephalitis
  • Genetic Code
  • Glycoproteins
  • Health Care
  • Health Services
  • Immunity
  • Immunization
  • Infection
  • Infectious Diseases
  • Interferon
  • Laboratory Animals
  • Lymphocytes
  • Proteins
  • Public Health
  • Rodents
  • Vaccines
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology