Validation of a Pan-Orthopox Real-Time PCR Assay for the Detection and Quantification of Viral Genomes from Nonhuman Primate Blood
Abstract
In 1980, smallpox disease was eradicated from nature and variola virus, the etiological agent of smallpox, was confined to two laboratories, one located in Russia (Moscow) later moved to VECTOR (Novosibirsk, Siberia) and one in the United States (CDC Atlanta). Vaccinations among the general public ceased shortly after the successful eradication campaign, resulting in an increasingly immunologically susceptible population. Because of the possibility of intentional reintroduction of the variola virus and the emergence of other pathogenic poxviruses, there is a great need for the development of medical countermeasures to treat poxvirus disease. It is highly likely that the U.S. FDA animal rule will necessary for regulatory approval of these interventions. Therefore, relevant animal models and the associated supporting assays will require development that can stand up to regulatory scrutiny.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 19, 2017
- Accession Number
- AD1035793
Entities
People
- Christopher Hartmann
- David A. B. Miller
- Donna Hering
- Eric M Mucker
- John Huggins
- Robert Fisher
- Wendy Giles
Organizations
- United States Army Medical Research Institute of Infectious Diseases