2014 Salish Kootenai College Equipment Grant
Abstract
Experiments to test pure protein on live human cells in vitro require the removal of all contaminants from protein expression. In order to do so, gel filtration is commonly employed as the highest level of purification. Gel filtration experiments can only be conducted using an appropriate purification machine. With this appropriate equipment, protein has been purified from a yeast expression system to explore the effects of that protein on human lung cells. The purpose of these experiments are to investigate the immune response when cells are stimulated with highly purified chilectin protein. Thus far, the protein has been successfully expressed and purified. The next steps will be to stimulate the cells and to isolate RNA for RTPCR to identify immune gene expression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 14, 2016
- Accession Number
- AD1038478
Entities
People
- Christina L. Rush
Organizations
- Salish Kootenai College