MASS SPECTROMETRY PROTEOMICS METHOD AS A RAPID SCREENING TOOL FOR BACTERIAL CONTAMINATION OF FOOD
Abstract
Every year in the United States there are over 48 million cases of foodborne illness. Traditional microbiological techniques require multiple enrichments using selective media for pathogen detection. Accurate identification of the offending pathogen is necessary to provide the most appropriate outbreak response and patient care. The mass spectrometry proteomics method (MSPM) does not require enrichment and is not affected by pathogens. The ability to use the MSPM to correctly classify whether or not food samples were contaminated with Salmonella enterica serotype Newport in this blinded pilot study resulted in a high level of sensitivity and specificity (>99 and 98.6 , respectively). The study involved mashed potato samples spiked at a concentration of 106 cfu/mL. These initial studies are encouraging and require further evaluation in more complex food matrices and at various pathogen concentrations to validate MSPM as a useful foodborne pathogen diagnostic tool.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2017
- Accession Number
- AD1039329
Entities
People
- Bernardo Delgado
- Candelaria C. Daniels
- Karyn A. Havas
- Mary M. Wade
- Patrick Mccubbin
- Rabih Jabbour
- Samir V. Deshpande
Organizations
- Edgewood Chemical Biological Center