Next-Generation Molecular Histology Using Highly Multiplexed Ion Beam Imaging (MIBI) of Breast Cancer Tissue Specimens for Enhanced Clinical Guidance

Abstract

We have completed the goals of the project timeline, in large part, in this second period of the grant funding. Specifically, we have developed the reagents, cell lines and tissues needed for the assessment of the dynamic range of protein quantitation. We have analyzed the lines with roughly 2/3rds of the planned assays, with the remaining 1/3rd in progress. We have optimized multiplexed IF for comparison to MIBI secondary ion mass spectrometry. And we have evaluated mRNA ISH probes for use in both IF and MIBI detection. The Stanford group in related but not funded by this proposal work has developed a second generation SIMS instrument which will enable higher throughput, and facilitate the further aims of the proposal in the coming grant periods.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2016
Accession Number
AD1039649

Entities

People

  • Garry P. Nolan

Organizations

  • Stanford University

Tags

DTIC Thesaurus Topics

  • Algorithms
  • Breast Cancer
  • Cell Line
  • Cells
  • Confocal Microscopy
  • Culture Techniques
  • Detection
  • Dynamic Range
  • Gene Expression
  • Ion Beams
  • Ions
  • Lymphocytes
  • Mass Spectrometry
  • Rocket Oxidizers
  • Spectrometers
  • Standards
  • Throughput

Readers

  • Clinical Trial Research.
  • Immunology and Pathology
  • Research Science/Academic Research