Testing of Ruggedized Antibodies within a Lateral Flow Immunoassay

Abstract

Antibody stability is a key quality parameter for a number of DoD customers who rely on effective and fielded hand-held immunoassays (HHAs). This project leverages antibody ruggedization efforts at U.S. Army Edgewood Chemical BiologicalCenter (ECBC) to determine whether the ruggedization of detection antibodies translates into improved and more fieldable assays. Capitalizing on the work performed at ECBC in collaboration with the Defense Advanced Research Projects Agency,Antibody Technology Program and the Defense Threat Reduction Agency, Ruggedized Antibody Program, this effort was projected to develop a lateral flow immunoassay against the ricin toxin and increase thermostability that enlists the use of asmartCAR reader device, which is capable of communicating results with a smartphone. Incorporating previously ruggedized ricin antibodies into fieldable HHAs potentially makes these assays more amenable to forward-deployed hazard detection and eliminates (or greatly reduces) the current requirement for 4 C cold-chain logistics.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2017
Accession Number
AD1041932

Entities

People

  • Alena M. Calm
  • Glen Ford
  • Hsiu Chen
  • Joe Ma
  • Kelley Betts
  • Randy Hofmann
  • Roy Thompson

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Antibodies
  • Biological Detection
  • Biological Warfare
  • Biological Warfare Agents
  • Department Of Defense
  • Department Of Homeland Security
  • Detection
  • Detectors
  • Immunoassay
  • Light Scattering
  • Logistics
  • Materials
  • Molecular Weight
  • Proteins
  • Test And Evaluation
  • Thermostability

Readers

  • Critical Infrastructure Protection in CBRN and WMD Threats.
  • Maritime Combat Support and Expeditionary Logistics.
  • Molecular and Cellular Biochemistry