Modeling Aggressive Medulloblastoma Using Human Induced Pluripotent Stem Cells

Abstract

In this project, we proposed to use neural cells derived from human induced pluripotent stem cells (iPSCs) to establish MYC-driven medulloblastoma (MB) model. This model will be used to identify the gene network regulated by MYC. We will also study the interaction between MYC and Atoh1, another essential transcription factor for MB development. Our results support that neural progenitors and neural stem cells induced from human induced pluripotent stem cells can be efficiently transformed by MYC oncogene to form aggressive brain tumors that recapitulate human group 3 MB. We further show that it is feasible to establish neurosphere cultures from these MB tumors to enrich brain tumor stem cells.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2017
Accession Number
AD1042682

Entities

People

  • Mingyao Ying

Tags

DTIC Thesaurus Topics

  • Blood-Brain Barrier
  • Cell Physiological Processes
  • Cells
  • Diseases And Disorders
  • Endothelial Cells
  • Gene Expression
  • Medical Personnel
  • Neoplasms
  • Neurons
  • Parkinson'S Disease
  • Proteins
  • Stem Cells
  • Transcription Factors

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular and Cellular Biology
  • Oncology

Technology Areas

  • Biotechnology