Multifunctional PSCA antibody fragments for PET and optical prostate cancer imaging
Abstract
The goals of this work are to develop engineered antibody fragments (cys-diabodies and cys-minibodies) for labeling with positron-emitting radionuclides and fluorescent dyes for multimodal imaging of prostate cancer. Previously, a novel cys-minibody recognizing PSCA (prostate stem cell antigen) was produced, conjugated, and radiolabeled with either I-124 or Zr-89. Targeting, imaging, and biodistribution studies in mouse models of prostate cancer confirmed excellent targeting and immunoPET imaging in PSCA-positive tumors in mice. This year, dual-labeling (with radionuclide and fluorescent dye) of the cys-minibody has been developed and evaluated in three different mouse models of prostate cancer. Furthermore, F-18 labeling has been established for a PSCA-specific cys-diabody and successfully imaged. A novel multifunctional linker has also been developed and dual F-18 PET/NIR fluorescence imaging has been achieved.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2017
- Accession Number
- AD1043779
Entities
People
- Anna M. Wu
Organizations
- University of California