Nanotechnology-Based Detection of Novel microRNAs for Early Diagnosis of Prostate Cancer
Abstract
Prostate cancer (PCa) is most commonly diagnosed and second leading cause of death in the US men, representing 8% of all male cancer deaths. Current gold standard for clinical detection of PCa consist of serum PSA test together with digital rectal examination (DRE). However, PSA screening predicted lower sensitivity (~21%) for PCa, while DRE is mostly linked with the patient discomfort due to painful procedure with a risk of slight bleeding and more importantly, poor sensitivity and specificity. Considering such limitations, there is an urgent need for a novel, non-invasive serum-based biomarker in conjunction with high-sensitive technique to differentiate indolent vs. aggressive prostate cancer (PCa). Emerging evidence suggests that several micro RNAs (miRs) are differentially expressed in tissues and sera of PCa patients and can potentially serve as biomarkers for disease progression and aggressiveness. However, quantification of ultra-low levels of serum miRNAs remains difficult with conventional quantification methods. Hence, present study was aimed at developing, optimizing and testing a novel DNA-gold nanoprobe (DNA-AuNPr)-based fluorescence assay for highly sensitive and specific quantification of miRNAs in serum samples. Herein, we successfully synthesized and characterized fluorescently-labeled DNA-gold nanoparticle probes (Nanoprobe) via bioconjugation approach. In parallel, metaanalysis and qPCR analysis were performed on prostate tissues excised from PTEN PCa mouse model and on PCa patient serum to identified differential expression level of miRNAs. We also analyzed expression patterns of various miRNAs (identified through meta-analysis of previously published reports) in mouse PCa tissues and in PCa patient serum compare to their respective controls. We observed a significant overexpression of miR-21 and miR-375 in both case.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2017
- Accession Number
- AD1043838
Entities
People
- Prakash Kshirsagar
Organizations
- University of Nebraska Omaha