Does the Loss of Stromal Caveolin-1 Remodel the Tumor Microenvironment by Activating Src-Mediated PEAK1 and PI3K Pathways

Abstract

This study describes a new mechanism of intercellular communication originating from extracellular vesicles (EVs). We demonstrate that in the context of prostate cancer, EV populations isolated from human patients harbor AKT1 and that AKT1 kinase activity is sustained in these particles, nominating them as active signaling platforms. Consistently, active AKT1 in circulating EVs from the plasma of metastatic prostate cancer patients is detected predominantly in large, tumor-derived EVs,termed large oncosomes (LO). LO internalization induces reprogramming of human normal prostate fibroblasts, as reflected by high levels of -SMA, IL6, and MMP9. In turn, LO reprogrammed normal prostate fibroblasts stimulate endothelial tube formation in vitro.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2016
Accession Number
AD1043933

Entities

People

  • Dolores Divizio
  • Mariana R. Sobreiro
  • Michael R Freeman
  • Wei Yang

Organizations

  • Cedars-Sinai Medical Center

Tags

DTIC Thesaurus Topics

  • Biological Factors
  • Blood
  • Cell Membrane
  • Cells
  • Cellular Structures
  • Confocal Microscopy
  • Endothelial Cells
  • Fibroblasts
  • Growth Factors
  • Mass Spectrometry
  • Medical Personnel
  • Neoplasms
  • Peptide Growth Factors
  • Peptides
  • Prostate
  • Prostate Cancer
  • Proteins

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Oncology (Cancer Research).
  • Prostate Cancer Biology.