Wound Healing in Mac-1 Deficient Mice
Abstract
Mac-1 (CD11b/CD18) is a macrophage receptor that plays several critical roles in macrophage recruitment and activation. Because macrophages are essential for proper wound healing, the impact of Mac-1 deficiency on wound healing is of significant interest. Prior studies have suggested that Mac-1-/- mice exhibit deficits in healing, including delayed wound closure in head and ear wounds. The current study examined whether Mac-1 deficiency influences wound healing in small excisional and incisional skin wounds. Three millimeter diameter full thickness excisional wounds and incisional wounds were prepared on the dorsal skin of Mac-1 deficient (Mac-1 -/-) and wild-type (WT) mice, and wound healing outcomes were examined. Mac-1 deficient mice exhibited a normal rate of wound closure, generally normal levels of total collagen, and nearly normal synthesis and distribution of collagens I and III. In incisional wounds, wound breaking strength was similar for Mac-1 -/- and WT mice. Wounds of Mac-1 deficient mice displayed normal total macrophage content, although macrophage phenotype markers were skewed as compared to WT. Interestingly, amounts of TGF-1 and its downstream signaling molecules, SMAD2 and SMAD3, were significantly decreased in the wounds of Mac-1 deficient mice compared to WT. The results suggest that Mac-1 deficiency has little impact on the healing of small excisional and incisional wounds. Moreover, the findings demonstrate that the effect of single genetic deficiencies on wound healing can markedly depend upon the wound model. These conclusions have implications for the interpretation of the many prior studies that utilize a single model system to examine wound healing outcomes in genetically deficient mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2017
- Accession Number
- AD1044062
Entities
People
- Alexander Y. Mitrophanov
- David Fine
- Jaques Reifman
- Jian Zhou
- Lin Chen
- Luisa A. Dipietro
- Sridevi Nagaraja
- Yan Zhao
Organizations
- Biotechnology High Performance Computing Software Applications Institute