Identifying Neurofibromin-Specific Regulatory Nodes for Therapeutic Targeting in NF1
Abstract
The overall goal of the project is to determine how neurofibromin is regulated, and to explore the hypothesis that loss of neurofibromin activity leads to up-regulation of specific receptors. We are building on our earlier discovery, that neurofibromin depends on the adapter protein SPRED1, to function, and we are utilizing the latest technical innovations including CRISPR technology to find genes that regulate neurofibromin SPRED function. To date we have demonstrated that oncogenic EGFR signaling disrupts Spred1-NF1 binding. Mass spectrometry was performed on cells overexpressing EGFRL858R to identify potential phosphorylation sites on Spred1 and NF1 that could disrupt Spred1-NF1 binding by steric hindrance.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2016
- Accession Number
- AD1044275
Entities
People
- Ellen Mercado
- Evan Markegard
- Frank P. Mccormick
- Osamu Tetsu
Organizations
- University of California, San Francisco