Identifying Therapeutics for Platinum-Resistant Ovarian Cancer by Next Generation Mechanotyping
Abstract
Treatment of high grade serous ovarian is initially effective in reducing the growth tumors, but cancer recurs in over 80% of ovarian cancer patients because cells become resistant to common, platinum-resistant chemotherapy drugs. There is a critical need for new drugs that target platinum-resistant cancer cells. We recently discovered that platinum-resistant ovarian cancer cells are more deformable than their drug-sensitive counterparts. We hypothesized that we could identify novel compounds that selectively target drug-resistant ovarian cancer cells by screening cells against libraries of small molecules using the novel Parallel Microfiltration (PMF) screening technology that we recently invented. In this first funding period, we have successfully advanced and integrated the PMF technology into the Molecular Shared Screening Resource at UCLA, thereby establishing the first facility for mechanotype screening. We have designed and optimized conditions for the mechanotype screen. Our proof-of-concept screen of cisplatin-resistant ovarian cancer cells against the Library of Pharmacologically Active Compounds(LOPAC) reveals several lead compounds. The pending studies will validate the effects of the lead compounds on cisplatin-resistant ovarian cancer cells, including cellular and molecular analyses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2017
- Accession Number
- AD1045516
Entities
People
- Amy Rowat
Organizations
- University of California