Alkaline Phosphatase for the Prevention of Intestinal and Renal Injury in a Rat Model of Cardiopulmonary Bypass with Deep Hypothermic Circulatory Arrest

Abstract

Purpose: To determine if alkaline phosphatase decreases intestinal and renal injury in a piglet model of cardiopulmonary bypass with DHCA, mediated in part through adenosine signaling pathways. Scope: Evaluate histologic (primary outcome), physiologic, and biomarker evidence of intestinal and kidney injury in this model with administration of escalating doses of bovine intestinal alkaline phosphatase or specific inhibitors of native alkaline phosphatase. Assess the role of downstream adenosine as a mediator of alkaline phosphatase effect. Major Findings: For the first reporting period, we had successfully established the model, demonstrating appropriate levels of physiologic disturbance (hemodynamic instability and lactic acidosis). Systemic interventions are nearly complete, at which time the initial analysis of the primary outcome will be performed for these groups.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2017
Accession Number
AD1045872

Entities

People

  • Jesse Davidson

Organizations

  • University of Colorado Boulder

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Acid-Base Imbalance
  • Adenosine
  • Animals
  • Biological Markers
  • Biomedical Research
  • Cardiac Arrest
  • Cardiovascular Diseases
  • Cardiovascular Surgery
  • Catheterization
  • Data Analysis
  • Department Of Defense
  • Diseases And Disorders
  • Heart Diseases
  • Instability
  • Intervention
  • Standards

Fields of Study

  • Medicine

Readers

  • Cardiovascular Physiology
  • Molecular and Cellular Biology