The Role of Tissue-Resident Donor T Cells in Rejection of Clinical Face Transplants

Abstract

Unlike solid organ transplants, face transplants have a unique immunological characteristic the presence of skin, which contains approximately 1 million T cell/cm2. A full face transplant is 600-700 cm2 in size and therefore, contains approximately 600-700 million donor T cells. The long-term persistence of donor T cells within facial allografts and the role of donor vs. recipient T cells in face transplant rejection are two critical issues that have never been studied. We plan to (i) quantify the number and diversity of donor vs. recipient T cells within face transplants over time using cutting edge high throughput TCR sequencing (HTS), (ii) identify pathogenic T cell clones implicated in rejection, (iii) identify if these pathogenic T cell clones are of donor vs. recipient, and (iv) determine if these pathogenic T cell clones are measurable in blood during episodes of rejection and could therefore serve as an earl and personalized biomarker of rejection.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2017
Accession Number
AD1046207

Entities

People

  • Bohdan Pomahač

Organizations

  • Brigham and Women's Hospital

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Allografts
  • Biological Markers
  • Biomedical Research
  • Blood
  • Cells
  • Electronic Mail
  • Governments
  • Health Services
  • Institutional Review Board
  • Local Governments
  • Lymphocytes
  • Medical Personnel
  • Professional Development
  • Rna Sequence Analysis
  • Throughput
  • Training
  • Transplants

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Neuroscience
  • Oncology

Technology Areas

  • Biotechnology