Global Identification of Disease-Associated Genes in Fragile X Cells
Abstract
The aim of the proposed study is to test the hypothesis that the Fragile X mental retardation protein (FMRP) prevents/resolves R loop formation to maintain genome stability. Specifically we propose that stable R loop formation impedes replication fork progression, resulting in DNA double strand breaks (DSBs), and that FMRP functions to prevent such replication transcription conflict. We have performed three biological replicate experiments to rigorously test if the Fragile X cell line produces more DSBs than the normal control. We also developed a yeast-based recombination assay to directly test the proposed function of FMRP in R loop prevention/resolution. Finally, we performed a ChIP-seq experiment to identify the chromatin binding sites of FMRP. We are working towards obtaining a short list of genes with overlapping DSBs, R loop forming sites and FMRP-binding sites. Potential disease correlation of these genes will then be assessed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2017
- Accession Number
- AD1046753
Entities
People
- Wenyi Feng
Organizations
- State University of New York