Potential Application of Viral Empty Capsids for the Treatment of Acute Lung Injury/Acute Respiratory Distress Syndrome

Abstract

The goal of this project has been to test the hypothesis that recombinant empty capsids of SV40, Virus Like Particles (VLPs), may attenuate ARDS, increasing survival and recovery from this severe clinical condition. We used the rat 2CLP (cecal ligation and two punctures) model for sepsis-induced ARDS. The VLPs, or vehicle (saline) control, were administered in 3 equal portions (total dose 0.3 mg/kg VLPs, in saline) for 3 days prior to the 2CLP operation. 2CLP was performed 24 hours later. While 100 the untreated rats died in 4 days, 75 of the VLP-treated ones survived. During the first 48 hours the treated rats presented similar symptoms and weight loss to the untreated ones. However afterwards their deterioration decelerated and they proceeded to full recovery. Lung pathology was normal in both treated and untreated rats sacrificed 24 hours after the 2CLP operation.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2017
Accession Number
AD1046772

Entities

People

  • Ariella Oppenheim

Organizations

  • Hebrew University of Jerusalem

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acute Respiratory Distress Syndrome
  • Bacteria
  • Blood
  • Blood Cells
  • Body Weight
  • Cells
  • Chemistry
  • Cytoskeleton
  • Health Services
  • Infection
  • Inflammation
  • Lung Diseases
  • Medical Personnel
  • Phagocytes
  • Sepsis
  • Therapy
  • Viral Structures

Readers

  • Immunology and Pathology
  • Infectious Disease/Epidemiology
  • Mathematics or Statistics